Scientific publications

Instituto Bernabéu is one of the leading fertility and assisted reproduction centres, with numerous publications in international journals.

The Association between Vaginal Dysbiosis and Reproductive Outcomes in Sub-Fertile Women Undergoing IVF-Treatment: A Systematic PRISMA Review and Meta-Analysis

Pathogens, 4 March 2021. Axel Skafte-Holm, Peter Humaidan, Andrea Bernabeu, Belén Lledó, Jorgen Skov Jensen and Thor Haahr.

Exploring the epigenetic profile of women with poor ovarian reserve

Fertility and Sterility, March '21. Rafael Bernabeu, José A. Ortíz, Belén Lledó. VER VIDEO

Sperm DNA fragmentation on the day of fertilisation is not associated with assisted reproductive technique outcome independently of gamete quality

Human Fertility, 27 Jan 2021. Jorge Ten, Jaime Guerrero, Ángel Linares, Adoración Rodríguez-Arnedo, Ruth Morales, Belén Lledó, Joaquín Llácer & Rafael Bernabeu.

Effect of ovarian stimulation on embryo aneuploidy and mosaicism rate

Systems Biology in Reproductive Medicine, Jan 2021. Alba Cascales , Belen Lledó , Jose A. Ortiz , Ruth Morales , Jorge Ten , Joaquin Llácer & Rafael Bernabeu

Consistent results of non-invasive pre-implantation genetic testing for aneuploidy (niPGT-A) of human embryos using two different techniques for chromosomal analysis

Reproductive BioMedicine Online, December 2020.  B. Lledo, R. Morales, J.A. Ortiz, A. Rodriguez-Arnedo, J. Ten, J.C. Castillo, A. Bernabeu, J. Llacer, R.Bernabeu. 

Expert consensus for primary management of reproductive health: a Delphi study

Expert consensus for primary management of reproductive health: a Delphi study. September 2020. Andrea Bernabeu, Concepcion Carratala-Munuera, Jose A. Quesada, Joaquin Llacer, Adriana Lopez-Pineda, Francisco Sellers, Vicente F. Gil-Guillen, Rafael Bernabeu, Kamila Cheikh-Moussa, Domingo Orozco-Beltran

Monozygotic twinning following embryo biopsy at the blastocyst stage

JBRA Assisted Reproduction Journal of Medical Sciences: September 2020. Rafael Sellers, Juan Carlos Castillo, Jorge Ten, Adoración Rodríguez, José A. Ortiz, Francisco Sellers, Joaquín Llácer, Rafael Bernabeu

Gonadotropin-releasing hormone agonist ovulation trigger—beyond OHSS prevention

Upsala Journal of Medical Sciences. Juan Carlos Castillo, Thor Haahr, María Martínez-Moya & Peter Humaidan

Sonographic Assessment of Uterine Fibroids and Adenomyosis

Gynaecological Ultrasound Scanning. Francisco Sellers López, Belén Moliner Renau and Rafael Bernabeu Pérez

Luteal phase stimulation versus follicular phase stimulation in poor ovarian responders: results of a randomized controlled trial

Reproductive Biology and Endocrinology. Joaquín Llácer, Belén Moliner, Lydia Luque, Andrea Bernabeu, Belén Lledó, Juan Carlos Castillo, Jaime Guerrero, Jorge Ten, Rafael Bernabéu.

Ovarian hyperstimulation syndrome following GnRH agonist trigger for final follicular maturation in a patient undergoing random start ovarian stimulation for egg-donation cycle with an inadvertent concomitant early pregnancy

Gynecological Endocrinology: December 2019. Juan Castillo, Joaquin Llacer, Ricardo Delgado, Jaime Guerrero, Rafael Bernabeu.

Comparison of the assisted reproductive technology outcomes between conventional IVF and ICSI with donor oocytes in normozoospermic patients

Human Fertility: November 2019. Jorge Ten, Patricia Peinado, Jaime Guerrero, Andrea Bernabeu, Joaquín Llácer, Domingo Orozco-Beltran, Concepcion Carratala-Munuera, Rafael Bernabeu.

The relevance of the individual screening for genetic variants in predicting ovarian response

Pharmacogenetics and Genomics: November 2019 - Volume 29 - Issue 9 - p 216–223. Belén Lledó; Joaquín Llácer; Laura Blanco; José A. Ortiz; Ruth Morales; Ana Fabregat; Jaime Guerrero; Rafael Bernabeu.

Effect of the vaginal microbiome on the pregnancy rate in women receiving assisted reproductive treatment

Journal of Assisted Reproduction and Genetics. August 2019. Andrea Bernabeu, Belén Lledo, Ma. Carmen Díaz, Francisca M. Lozano, Vicente Ruiz, Ana Fuentes, Adriana Lopez-Pineda, Belen Moliner, Juan Carlos Castillo, Jose Antonio Ortiz, Jorge Ten, Joaquin Llacer, Concepcion Carratala-Munuera, Domingo Orozco-Beltran, Jose A. Quesada, Rafael Bernabeu.

The use of fluorescence in situ hybridization analysis on sperm: indications to perform and assisted reproduction technology outcomes

Journal of Assisted Reproduction and Genetics. August 2019. Zaida Sarrate, Joan Blanco, Fernando Marina-Rugero, Juan Manuel Moreno-García, Miguel Ruiz-Jorro, Rafael Lafuente-Varea, Fernando Graña-Zanón, Rocío Núñez-Calonge, Jorge Ten, Joaquín Rueda.

Comprehensive mitochondrial DNA analysis and IVF outcome

Human Reproduction Open, pp. 1–9, 2018. November 2018. B. Lledo, J.A. Ortiz, R. Morales, E. García-Hernández, J. Ten, A. Bernabeu, J. Llácer, R. Bernabeu.

Clinical outcomes in elective vitrification of embryos

Revista Oficial de la Sociedad Española de Ginecología y Obstetricia, Prog Obstet Ginecol 2018;61(5):438-443. November 2018. Rafael Sellers Gil, Jorge Ten Morro, Mª Carmen Tió Marquina, Francisco Sellers López, and Rafael Bernabeu Pérez.

Prevalence of candidate single nucleotide polymorphisms on p53, IL-11, IL-10, VEGF and APOE in patients with repeated implantation failure (RIF) and pregnancy loss (RPL)

Human Fertility. October 2018. Azahara Turienzo, Belén Lledó, José A. Ortiz, Ruth Morales, Juan Sanz, Joaquín Llácer & Rafael Bernabeu.

A pharmacogenetic approach to improve low ovarian response: The role of CAG repeats length in the androgen receptor gene

European Journal of Obstetrics & Gynecology and Reproductive Biology. Volume 227, August 2018, Pages 41-45. B.Lledó, J.Llácer, JA.Ortiz, B.Martinez, R.Morales, R.Bernabeu.

Does embryo mosaicism affect clinic results in assisted reproduction cycles?

Young Investigator Award winner.

Abstracts for 2017 Foundation for Reproductive Medicine Translational Reproductive Biology and Clinical Reproductive Endocrinology Conference. 

J Assist Reprod Genet (2017) 34:1385–1402. October 2017. Andrea Bernabeu, José Antonio Ortiz, Belén Lledó, Ruth Morales, Elena Fernández, Jorge Ten, Rafael Bernabeu.

Estudio del volumen y la vascularización trofoblástica mediante power doppler tridimensional en la novena semana en gestaciones espontáneas y tras técnicas de reproducción asistida

Elsevier. 154-161. Junio 2017. Francisco Sellers, Belén Moliner, Ana Palaciosa, Rafael Sellers, Joaquín Llácer y Rafael Bernabeu.

Human sperm motility, capacitation and acrosome reaction are impaired by 2- arachidonoylglycerol endocannabinoid.

Histology and Histopathology. 11-911. June 2016. M.M. Francou, J.L. Girela, A. De Juan, J. Ten, R. Bernabeu, J. De Juan. 

Implantation potential of mosaic embryos.

Systems Biology in Reproductive Medicine. Vol63, NO.3, 206-208. April 2017. B. Lledó, R. Morales, JA. Ortiz, H. Blanca, J. Ten, J. Llácer, R. Bernabeu. 

Congenital anomalies after assisted reproduction techniques and their correlation with embryonic quality

Revista Iberoamericana de Fertilidad. Vol. 33 nº 4. November 2016. Francisco Sellers López, Belén Moliner Renau, Jorge Ten Morro, Jaime Guerrero Villena, Ana Palacios Marqués, Rafael Bernabeu Pérez.

Study of placental vascularization using 3D Power Doppler on week 20 of spontaneous pregnancies and after assisted reproductive techniques

Revista Iberoamericana de Fertilidad. Vol. 33 nº 4. October 2016. Francisco Sellers López, Belén Moliner Renau, Ana Palacios Marqués, Rafael Bernabeu Pérez.

Chromosomal polymorphic variants increase aneuploidies in male gametes and embryos

Systems Biology in Reproductive Medicine. Vol. 62,  Issue 5, pp 317-324. August 2016. Ruth Morales, Belén Lledó, José A. Ortiz, Jorge Ten, Joaquin Llácer & Rafael Bernabeu.

Clinical efficacy of recombinant versus highly purified follicle-stimulating hormone according to follicle-stimulating hormone receptor genotype

Pharmacogenetics Genomics. Vol 26 (6), 288-93. June 2016.  Belén Lledó, Patricia Dapena, Jose A. Ortiz, Ruth Morales, Joaquín Llacer and Rafael Bernabeu

Can reproductive medicine help to understand the aetiopathogenesis of pre-eclampsia?

Reproductive Medicine and Clinical Embryology (MEDRE). Vol. 03, 1-3. 2016. F. Sellers, J. Llácer y R. Bernabeu. 

Metabolites involved in cellular communication among human cumulus-oocyte-complex and sperm during in vitro fertilization

Reproductive Biology and Endocrinology. November 2015. M.J. Gómez, E.M. García, J. Guerrero, S. Medina, M. J. Izquierdo, A. Gil, J. Orduna, M. Saviron, J. Ten, R. Bernabeu, M. Avilés, L. González. 

Androgen receptor CAG repeat length is associated with ovarian reserve but not with ovarian response.

RBMOnline. Vol. 29, 509-515. October 2014. B. Lledo, J. Llácer, A. Turienzo, J.A. Ortiz, J. Guerrero, R. Morales, J. Ten, R. Bernabeu.

Pharmaceutical options for triggering of final oocyte maturation in ART.

BioMed Research International. vol. 2014, Article ID 580171, 7 pages, 2014. doi:10.1155/2014/580171. July 2014. J. C. Castillo, P. Humaidan, and R. Bernabeu

Pharmacogenetics of ovarian response

 Pharmacogenomics. 15 (6), pp 885-893. June 2014. B. Lledó, J. A. Ortíz, J. Llácer, R. Bernabeu

A multicenter prospective study to assess the effect of early cleavage on embryo quality, implantation, andlive-birth rate

Fertility and Sterility Vol. 104, Issue 4. Pages 981-987. April 2014. Maria José de los Santos, Ph.D.,Gemma Arroyo, Ph.D.,Ana Busquet, Ph.D.,Gloria Calderon, Ph.D.,Jorge Cuadros, Ph.D.,Maria Victoria Hurtado de Mendoza, Ph.D.,Marta Moragas, Ph.D.,Raquel Herrer, Ph.D., Agueda Ortiz, Ph.D.,Carme Pons, M.Sc.,Jorge Ten, Ph.D.,Miguel Angel Vilches, Ph.D., and Maria Jose Figueroa, Ph.D., for the ASEBIR Interest Group in Embryology.

Study of structural damage after sperm immobilisation prior to intracytoplasmic sperm injection in teratozoospermic males.

International Journal of Andrology. Volume 12 (1):10–15. January-March 2014. María José Gómez-Torresa, Eva María García, Jaime Guerrero, Jose Luis Girela, Jorge Tena, Rafael Bernabeub y Joaquín De Juan. 

First place prize for original work. 8th Edition of Angel Bayo Awards. ASESA (Spanish Andrology Society). June 2015.

Negative effect of P72 polymorphism on p53 gene in IVF outcome in patients with repeated implantation failure and pregnancy loss

Journal of Assisted Reproduction and Genetics. November 2013.B. Lledó, A. Turienzo, J.A. Ortíz, R. Morales, J. Ten, J. Llácer, and R. Bernabeu.

Capacitation and acrosome reaction changes α-tubulin immunodistribution in human spermatozoa

RBMOnline. October 2013. M.M. Francou, J. Ten, J. De Juan and R. Bernabeu.

Characterization of a balanced complex chromosomal rearrangement carrier ascertained through a fetus with dup15q26.3 and del5p15.33: case report

Human Fertility. August 2013. B. Lledó, J. A. Ortiz, R. Morales, I. Manchón, F. Galán, A. Bernabeu and R. Bernabeu.

Effect of follicle-stimulating hormone receptor N680S polymorphism on the efficacy of follicle-stimulating hormone stimulation on donor ovarian response.

 Pharmacogenetics and Genomics. March 2013. B. Lledo, J. Guerrero, A. Turienzo, J.A. Ortiz, R. Morales, J. Ten, J. Llacer and R. Bernabeu.

Uterine arteriovenous malformation

British Medical Journal (BMJ) Case Rep 2013.doi: 10.1136/bcr-2012-008443. February 2013.F. Sellers, A. Palacios, B. Moliner, R. Bernabeu.

New Approach for Chemometric Analysis of Mass Spectrometry Data

  • 02/01/13

Analytical Chemistry. Vol.85 (6).pp 3053-3058. February 2013. F.C. Marhuenda-Egea, R. D. Gonsálvez-Álvarez, B. Lledó-Bosch, J. Ten, R. Bernabeu.

Androgen receptor Polymorphisms are associated with poor ovarian response

Fertility and Sterility Vol. 98, Issue 3, Supplement, Page S114. September 2012. ASRM Abstracts. J. Llácer, J.A. Ortiz, J. Guerrero, B. Lledó, J. Ten, R. Bernabeu.2nd place ASRM award for the best conference poster.

Sperm deoxyribonucleic acid fragmentation level, as measured by tunel test, is not related with the IVF outcome in good prognosis women.

Fertility and Sterility Vol. 98, Issue 3, Supplement, Page S150. September 2012. ASRM Abstracts. B. Lledó, R. Morales, A. Rodríguez, J. Ten, R. Bernabeu.

High ovarian stimulation doesn't impair embryo quality. Clinical evidence using comparative model with shared egg donors

Fertility and Sterility Vol. 98, Issue 3, Supplement, Pages S171-S172. September 2012. ASRM Abstracts. J. Llácer, B. Moliner, A. Rodríguez, J. Guerrero, J. Ten, R. Bernabeu.

Effect of polymorphism in the promoter estrogen receptor alpha on sperm count

Fertility and Sterility Vol. 98, Issue 3, Supplement, Page S243. September 2012.ASRM Abstracts. B. Lledó, A. M. Fabregat, R. Morales, J. A. Ortiz, J. Llácer, R. Bernabeu.

Intermediate and normal sized CGG repeat on the FMR1 gene does not negatively affect donor ovarian response

Human Reproduction Vol. 27, No. 2. pp 609-614. February 2012. B. Lledo, J. Guerrero, J.A. Ortiz, R. Morales, J. Ten, J. Llácer, J. Giménez, R. Bernabeu.

Correlations between Different Heavy Metals in Diverse Body Fluids: Studies of Human Semen Quality

Advances in Urology. Vol. 2012, Art. ID 420893, 11 pages. 2012. L. Mínguez-Alarcón, J. Mendiola, M. Roca, J.J. López-Espín, J.J. Guillén, J.M. Moreno, S. Moreno-Grau, M.J. Martínez-García, N. Vergara-Juárez, B. Elvira-Rendueles, A. García-Sánchez, J. Ten, R. Bernabeu, A.M. Torres-Cantero.

Relationships between heavy metal concentrations in three different bodily fluids and male reproductive parameters: a pilot study

Environmental Health. 10:6. 2011. J. Mendiola, J.M. Moreno, M. Roca, N. Vergara-Juárez, M.J. Martínez-García, A. García-Sánchez, B. Elvira-Rendueles, S. Moreno-Grau, J.J. López-Espín, J. Ten, A.M. Torres-Cantero, R. Bernabeu.

Improving human embryos selection in IVF: non-invasive metabolomic and chemometric approach

Metabolomics (2011) 7:247-256. 2011. F. C. Marhuenda-Egea, R. Gonsálvez-Álvarez, E. Martínez-Sabater, B. Lledó, J. Ten, R. Bernabeu.

Analysis of Placental Vascularization by Means of 3D Power Doppler in Women Pregnant Following Oocyte Donation

Reproductive Sciences. Vol. 17, No. 8, pp 754-759. August 2010. F. Sellers López, D. Orozco-Beltrán, V. Gil-Guillen, J.M. Lozano, A. Palacios, R. Bernabeu.

The paternal effect of chromosome translocation carriers observed from meiotic segregation in embryos

Human Reproduction. Vol. 25, No. 7, pp 1843-1848. July 2010. B. Lledó, J.A. Ortiz, R. Morales, J. Ten, P.E. de la Fuente, C. García-Ochoa, R. Bernabeu

A crucial step in assisted reproduction technology: human embryo selection using metabolomic evaluation

Fertility and Sterility. Vol. 94. No. 2. 772-774, July 2010.F.C. Marhuenda-Egea, E. Martínez-Sabater, R. Gonsálvez-Álvarez, B. Lledó, J. Ten, R. Bernabeu.

A low intake of antioxidant nutrients is associated with poor semen quality in patients attending fertility clinics

Fertility and Sterility. Vol. 93, No. 4, March 1, 2010. J. Mendiola, A. M. Torres-Cantero, J. Vioque, J. M. Moreno-Grau, J. Ten, M. Roca, S. Moreno-Grau, R. Bernabeu.

Food intake and its relationship with semen quality: a case-control study

Fertility and sterility Vol. 91, Nº3, March 2009. J. Mendiola, A.M. Torres-Cantero, J.M. Moreno-Grau, J. Ten, M. Roca, S. Moreno Grau, R. Bernabeu.

Exposure to environmental toxins in males seeking infertility treatment: a case-controlled study

Reproductive BioMedicine Online. Vol. 16, No 6, 842-850. 2008.J. Mendiola, A.M. Torres-Cantero, J.M. Moreno-Grau, J. Ten, M. Roca, S. Moreno-Grau, R. Bernabeu.

Occupational and Lifestyle Exposures on Male Infertility: A Mini Review

The Open Reproductive Science Journal. Vol. 1, 16-21. 2008. J. Ten, J. Mendiola, A.M. Torres-Cantero, J.M. Moreno-Grau, S. Moreno-Grau, M. Roca, J. Romero and R. Bernabeu.

Preimplantation genetic diagnosis of X-linked retinoschisis

Reproductive BioMedicine Online Vol. 16, No. 6, 886-892. 2008. B. Lledó, J. Ten, D. Rodríguez-, J. Llácer, R. Bernabeu.

Preimplantation genetic diagnosis of X-linked adrenoleukodystrophy with gender determination using multiple displacement amplification

Fertility and Sterility. Vol. 88, No. 5, 1327-1333. November 2007. B. Lledo, R. Bernabeu, J. Ten, F.M. Galan, L. Cioffi.

Sperm immobilized before intracytoplasmic sperm injection undergo ultrastructural damage and acrosomal disruption

Fertility and Sterility. Vol. 88, No.3, 702-704. September 2007. M.J. Gomez-Torres, J. Ten, J.L. Girela, J. Romero, R. Bernabeu, J. De Juan.

Development of Assisted Reproduction. Past, Present and Future of Technologies

Bioforum Europe. Vol. 11, 64-65. 2007. B. Lledó, J. Llácer, J. Ten and R. Bernabeu.

Donor oocyte dysmorphisms and their influence on fertilization and embryo quality

Reproductive BioMedicine Online. Vol. 14. No. 1, 40-48. January 2007.J. Ten, J. Mendiola, J. Vioque, J. de Juan, R. Bernabeu.

Preimplantation genetic diagnosis of Marfan syndrome using multiple displacement amplification

Fertility and Sterility. Vol. 86, No. 4, 949-955. October 2006. B. Lledó, J. Ten, F.M. Galán, R. Bernabeu.

Indomethacin effect on implantation rates in oocyte recipients

Human Reproduction. Vol. 21, No. 2. pp. 364-369. February 2006. M. Roca, A. Torres, J. Ten, R. Bernabeu.

Analysis of factors influencing pregnancy rates in homologous intrauterine insemination

Fertility and Sterility Vol. 81, No. 5, 1308-1313. May 2004. G. Ibérico, J. Vioque, N. Ariza, J.M. Lozano, M. Roca, J. Llacer, R. Bernabeu.

Follicular fluid markers of oocyte developmental potential

Human Reproduction. Vol. 17, No.4. pp 1017-1022. April 2002. C. Mendoza, E. Ruiz-Requena, E. Ortega, N. Cremades, F. Martínez, E. Greco, J. Tesarik and R. Bernabeu.

Historical comment on the debate: embryo implantation is the rubicon for the GnRH antagonist, and answer

Human Reproduction. Vol. 16, No. 10, p. 2247. October 2001. R. Bernabeu.

Developmental potential of elongating and elongated spermatids obtained after in-vitro maturation of isolated round spermatids

Human Reproduction. Vol. 16, No.9. pp. 1938-1944, September 2001. N. Cremades, M. Sousa, A. Barros and R. Bernabeu.

In-vitro maturation of round spermatids using co-culture on Vero cells

Human Reproduction. Vol. 14, No. 5. pp. 1287-1293. May 1999.N. Cremades, A. Barros, M. Sousa, R. Bernabeu.

Effects of Agonist dose on in-vitro fertilization

Human Reproduction. Vol.13. No. 10. pp. 2973-2974. October 1998. C. Álvarez and R. Bernabeu.

Successful pregnancy after spermatid injection

Human Reproduction Vol. 13, No.7. pp. 1898-1900. July 1998. N. Cremades, K. Takahashi, M. Sousa, R. Bernabeu.

Influence of gonadotrophin-releasing hormone agonist total dose in the ovarian stimulation in the long down-regulation protocol for in-vitro-fertilization

Human Reproduction. Vol 12. No 11. Pp. 2366-2369. November 1997. C. Álvarez, N. Cremades, N. Blasco and R. Bernabeu.

Deletion of chromosome 13: 46, XY del (13) (q14->qter) after in vitro fertilization and tubal embryo transfer

  • 07/01/96

Journal of Assisted Reproduction and Genetics, Vol. 13, No 6. July 1996. M. Bonada, N. Cremades, F. Galán, R. Bernabeu.

Sex selection may be inadvertently performed in in-vitro fertilization-embryo transfer programmes

Human Reproduction. Vol. 10, No 11. pp. 2992-2998. November 1995. JJ. Tarín, A. Baviera, M. Bonada, A. Cano and R. Bernabeu.